Frost Tolerance

In Australia and other cereal growing regions with a Mediterranean climate, frost events at flowering can cause sterility, leading to extreme yield reductions, as well as damaged seeds. In Victoria and South Australia alone, frost causes annual losses in wheat and barley productivity of around $95.8m and $33.6m, respectively. Severe winter temperatures experienced in some northern hemisphere regions can also kill plants at the hardier vegetative stage. Periods of cold but non-freezing temperatures can also reduce fertility.

Plants in frost chamber

Frost affected Barley heads


  1. Isolate genes determining natural frost and cold tolerance.
  2. Characterize the molecular and physiological mechanisms of frost tolerance.
  3. Identify strategies for genetic engineering of frost tolerance in cereals.
  4. Generate markers and knowledge to assist conventional frost tolerance breeding.

Experimental Strategy

The Waite barley breeding group identified regions of the 2H and 5H barley chromosomes determining varietal differences in frost tolerance. Fine-mapping and cloning of the underlying genes is being aided by the conservation in gene content and order between barley and the sequenced rice genome. A state-of-the-art frost simulation chamber housed in the Australian Genome Research Facility (AGRF) on campus enables evaluation of frost tolerance levels. Genes are being functionally evaluated using barley and wheat transformation.

Current research activities

Current project:

Factors affecting performance of chamber-based frost tolerance assays, and correlation with tolerance in the field.


Dr Nick Collins

 Selected Publications

  • Chen A, Gusta LV, Brûlé-Babel A, Leach R, Baumann U, Fincher GB, Collins NC (2009). Varietal and chromosome 2H locus-specific frost tolerance in reproductive tissues of barley (Hordeum vulgare L.) detected using a frost simulation chamber. Theoretical and Applied Genetics 119: 685-694.
  • Chen A, Baumann U, Fincher GB, Collins NC (2009). Flt-2L, a locus in barley controlling flowering time, spike density, and plant height. Functional and Integrative Genomics 9: 243-254.
  • Chen A, Reinheimer J, Brûlé-Babel A, Baumann U, Pallotta M, Fincher GB, Collins NC (2009). Genes and traits associated with chromosome 2H and 5H regions controlling sensitivity of reproductive tissues to frost in barley. Theoretical and Applied Genetics 118: 1465-1476.
  • Chen A, Brûlé-Babel A, Baumann U, Collins NC (2009). Structure-function analysis of the barley genome: the gene-rich region of chromosome 2HL. Functional and Integrative Genomics 9: 67-79.
  • Collins NC, Tardieu F, Tuberosa R (2008) Quantitative trait loci and crop performance under abiotic stress: Where do we stand? Plant Physiology 147: 469-486.
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