The technology - with a view to developing 'technology service centres' for the Nutriome Network
 

Structural genomics and quantitative genetics

Functional Genomics

Whole plant physiology

Cellular techniques - including characterisation of transporter genes

Microscopy and Imaging

 

 

Field studies

Breeding approaches

  Structural genomics and quantitative genetics

Mapping

Synteny

Genome structure

Generation of mutants and mutant libraries

  Functional Genomics

Transcriptomics - ESTs, microarrays, etc.

Proteomics

Metabolomics

Bioinformatics

  Whole plant physiology

Growth studies

Plant Nutrient Analyses - ICP-AES, ICP-MS, membrane inlet mass spectrometry of diffusible ions

Stable isotopes

Radioactive tracer measurements

Transporter kinetic properties

  Cellular techniques - including characterisation of transporter genes

Electrophysiology

Patch clamping, planar lipid bilayers

MIFE

Other cellular methods for measuring fluxes (e.g. pressure probe,stopped flow spectrophotometry)

Micro-injection of fluorescent tracers

Isolation of transporter genes and heterologous expression systems

Localisation of expression - Q-PCR, promoter-reporter fusions, in situ hybridisation, etc

Protein localisation - immunocytochemistry, immunofluorescence, protein-GPF fusions

Nano-SIMS

  Microscopy and Imaging

Confocal scanning laser microscopy

Scanning and Transmission electron microscopy

Protein structure - crystallography, NMR

 

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