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Damian
Drew
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PhD
Student
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Mailing Address
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Contact Information
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Australian Centre for Plant Functional Genomics
University of Adelaide
Waite Campus
Glen Osmond,
South Australia 5064 Australia
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Australian Centre for Plant Functional
Genomics
Hartley Grove, Urrbrae
Postal PMB 1, Glen Osmond SA 5064
p +61
8 8303 6501 f +61 8 8303 7102
e
damian.drew@acpfg.com.au
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Qualifications
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Bachelor
of Medical and Pharmaceutical Biotechnology with Honours – University of South Australia (2003)
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Expertise and Research Interests
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Previous research
2003 (Honours project)
Investigation into post-translational
control of the von Hippel-Lindau tumour
suppressor protein, an E3 ubiquitin ligase that mediates the regulated ubiquitination, and subsequent degradation, of
hypoxia inducible factor (HIF1a), a angiogenesis
inducing transcription factor.
Experience and expertise gained includes the culture of human
embryonic kidney cells (HEK293T), transient and stable expression of HA-
and His-tagged proteins, partial purification via immobilized metal
affinity chromatography, immunoprecipitation
assays, poly-acrylamide gel electrophoresis and immunoblotting,
narrow-range isoelectric focusing 2D
electrophoresis and in-vivo UV cross-linking.
2004-2005
Cloning, expression, purification, qPCR expression profiling and enzymic analysis of the
oxidative stress protein, monodehydroascorbate reductase from the moss, Physcomitrella
patens. Experience and expertise gained includes the growth and
maintenance of Physcomitrella patens, molecular cloning of genes
including the addition of His-tags and restriction sites, site directed
mutagenesis, enzyme kinetic assays, calculation of Michaelis-Menten
constants, spectrophometric protein analysis,
HPLC and MS based identification of enzyme co-factors.
Current research interests
Structural and functional analysis of
the sodium and calcium transporting P-type ATPases, PpENA1 and AtECA1
from Physcomitrella patens and Arabidopsis thaliana
respectively. Identification of ion binding sites via homology modelling
and yeast complementation studies, and site directed mutagenesis of
numerous residues of interest. Use of gateway and TOPO cloning systems
and galactose inducible gene expression using the pYES3-DEST vector in
the salt-sensitive B31 strain of S. cerevisiae.
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Memberships
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Member of the Australian Society of Biochemistry and
Molecular Biology
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Publications
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Lunde C, Baumann U, Shirley NJ, Drew DP, Fincher GB, Gene structure and expression pattern
analysis of three monodehydroascorbate reductase (Mdhar) genes in Physcomitrella patens: Implications
for the evolution of the MDHAR family in plants, Plant Mol.
Biol., In Press.
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Funding Received
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ACPFG
PhD scholarship
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Languages
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English
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Reading, Writing and
Speaking
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Profile Details
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Last Updated:
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21/11/05
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Search Keywords
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monodehydroascorbate
reductase, MDHAR,
oxidative
stress, enzyme kinetics, PpENA1,
AtECA1,
P-type ATPase, ion binding,
homology
modelling, mutagenesis
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Individual
Expertise Profile, Copyright 2005.
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